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postsynaptic density protein 95 psd95  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc postsynaptic density protein 95 psd95
    Postsynaptic Density Protein 95 Psd95, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/postsynaptic+density+protein+95+psd95/pm41749086-54-46-60
    Average 86 stars, based on 1 article reviews
    postsynaptic density protein 95 psd95 - by Bioz Stars, 2026-11
    86/100 stars

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    Incubation:

    Article Title: Guilu Erxian Jiao remodels dendritic spine morphology through activation of the hippocampal TRPC6-CaMKIV-CREB signaling pathway and suppresses fear memory generalization in rats with post-traumatic stress disorder.
    Article Snippet: Ethnopharmacological relevance: Guilu Erxian Jiao (GLEXJ) is a renowned traditional Chinese herbal formula used to tonify the kidney.. It is employed to treat psychiatric disorders, and alleviate memory impairment, cognitive dysfunction, and behavioral disorders.. Modern pharmacological studies have demonstrated GLEXJ’s ability to significantly inhibit the fear response in post-traumatic stress disorder (PTSD) and facilitate the extinction of fear memory.

    Article Title: Metformin activates the PI3K/AKT/BDNF axis to attenuate postoperative cognitive dysfunction.
    Article Snippet: Postoperative cognitive dysfunction (POCD) is a prevalent neurocognitive complication of anesthesia and surgery.. Metformin, a widely used antidiabetic drug, has neuroprotective properties and improves cognitive impairment and memory deficits.. However, the mechanisms underlying its action in improving cognitive dysfunction after anesthesia and surgery remain unclear.

    Article Title: Nr4a1 regulates microglia overactivation-mediated neuroinflammation to ameliorate cognitive impairment in trigeminal neuralgia rats
    Article Snippet: BV2 and HT22 cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100 (P0096; Beyotime). .. Cells were blocked with 2% bovine serum albumin and incubated overnight at 4 °C with primary antibodies against Iba1 (1:300; ab283319, Abcam), p-Akt2 (1:200; 8599, Cell Signaling Technology), or Postsynaptic density protein 95 (PSD95) (1:200; 3409, Cell Signaling Technology). .. Subsequently, cells were incubated in the dark for 1 h with fluorescent secondary antibodies Alexa Fluor ® 555 goat anti-mouse IgG (1:200; ab150118, Abcam) or Alexa Fluor ® 647 goat anti-rabbit IgG (1:200; ab150079, Abcam), followed by staining with 4’,6-diamidino-2-phenylindole.

    Article Title: GDNF attenuates a-synuclein aggregation-induced damage to VTA-NAc dopaminergic transmission and alleviates depression-like behaviors in mice
    Article Snippet: .. The membranes were closed for 2 h at room temperature (20–25 °C) with 5% skimmed milk, which subsequently, were incubated overnight at 4 °C with specified primary antibodies (α-syn, Abcam, Cat# ab138501; TH, Santa, Cat# sc-374048; p-α-syn, Thermo Fisher Scientific, Cat# PA5-37740; synaptosomal-associated protein 25 (SNAP-25), Santa, Cat# sc-20038; postsynaptic density protein 95 (PSD95), CST, Cat# 3450 S; D2R, Santa, Cat# sc-5303; D1R, CST, Cat# #79777T; DAT, Santa, Cat# sc-32259; GDNF, Affinity, Cat# DF7727; glyceraldehyde-3-phosphate dehydrogenase (GAPDH), huabio, Cat# ET1601-4; β-actin, Proteintech, Cat# 66009-l-lg). .. The membranes were washed three times with wash buffer, and then incubated with corresponding secondary antibodies (goat anti-rabbit IgG secondary antibody: Cat# 926-32211, goat anti-mouse IgG secondary antibody: Cat# 926-68070, LI-COR, USA) for 2 h at room temperature.

    Article Title: CB2R-mediated GPX4 stabilization: a new avenue for treating cognitive impairment in elderly migraine patients.
    Article Snippet: .. Both samples were washed with phosphate-buffered saline (PBS), blocked with 5% bovine serum albumin (BSA) in PBS for 1 h, and incubated at 4 °C overnight with primary antibodies (ionized calcium-binding adapter molecule 1 (IBA-1), GPX4, interleukin-1β (IL-1β), CB2R, calcitonin gene-related peptide (CGRP), C-fos, synaptophysin (SYP), postsynaptic density protein 95 (PSD95), inducible nitric oxide synthase (iNOS), ubiquitin (UB), purchased from Cell Signaling Technology or Santa Cruz) in 3% BSA/PBS. .. After PBS washing, samples were incubated with secondary antibodies (rabbit 488, mouse 555: 1:500; Invitrogen) for 2 h at room temperature, followed by 4’,6-diamidino-2-phenylindole (DAPI) (1:2000; Thermo Fisher) staining for 10 min and PBS washes.

    Article Title: CB2R-mediated GPX4 stabilization: a new avenue for treating cognitive impairment in elderly migraine patients
    Article Snippet: .. Both samples were washed with phosphate-buffered saline (PBS), blocked with 5% bovine serum albumin (BSA) in PBS for 1 h, and incubated at 4 °C overnight with primary antibodies (ionized calcium-binding adapter molecule 1 (IBA-1), GPX4, interleukin-1β (IL-1β), CB2R, calcitonin gene-related peptide (CGRP), C-fos, synaptophysin (SYP), postsynaptic density protein 95 (PSD95), inducible nitric oxide synthase (iNOS), ubiquitin (UB), purchased from Cell Signaling Technology or Santa Cruz) in 3% BSA/PBS. .. After PBS washing, samples were incubated with secondary antibodies (rabbit 488, mouse 555: 1:500; Invitrogen) for 2 h at room temperature, followed by 4’,6-diamidino-2-phenylindole (DAPI) (1:2000; Thermo Fisher) staining for 10 min and PBS washes.

    Article Title: GDNF attenuates a-synuclein aggregation-induced damage to VTA-NAc dopaminergic transmission and alleviates depression-like behaviors in mice.
    Article Snippet: .. The membranes were closed for 2 h at room temperature (20–25 °C) with 5% skimmed milk, which subsequently, were incubated overnight at 4 °C with specified primary antibodies (α-syn, Abcam, Cat# ab138501; TH, Santa, Cat# sc-374048; p-α-syn, Thermo Fisher Scientific, Cat# PA5-37740; synaptosomal-associated protein 25 (SNAP-25), Santa, Cat# sc-20038; postsynaptic density protein 95 (PSD95), CST, Cat# 3450 S; D2R, Santa, Cat# sc-5303; D1R, CST, Cat# #79777T; DAT, Santa, Cat# sc-32259; GDNF, Affinity, Cat# DF7727; glyceraldehyde-3-phosphate dehydrogenase (GAPDH), huabio, Cat# ET1601-4; β-actin, Proteintech, Cat# 66009-l-lg). .. The membranes were washed three times with wash buffer, and then incubated with corresponding secondary antibodies (goat anti-rabbit IgG secondary antibody: Cat# 926-32211, goat anti-mouse IgG secondary antibody: Cat# 926-68070, LI-COR, USA) for 2 h at room temperature.

    Saline:

    Article Title: CB2R-mediated GPX4 stabilization: a new avenue for treating cognitive impairment in elderly migraine patients.
    Article Snippet: .. Both samples were washed with phosphate-buffered saline (PBS), blocked with 5% bovine serum albumin (BSA) in PBS for 1 h, and incubated at 4 °C overnight with primary antibodies (ionized calcium-binding adapter molecule 1 (IBA-1), GPX4, interleukin-1β (IL-1β), CB2R, calcitonin gene-related peptide (CGRP), C-fos, synaptophysin (SYP), postsynaptic density protein 95 (PSD95), inducible nitric oxide synthase (iNOS), ubiquitin (UB), purchased from Cell Signaling Technology or Santa Cruz) in 3% BSA/PBS. .. After PBS washing, samples were incubated with secondary antibodies (rabbit 488, mouse 555: 1:500; Invitrogen) for 2 h at room temperature, followed by 4’,6-diamidino-2-phenylindole (DAPI) (1:2000; Thermo Fisher) staining for 10 min and PBS washes.

    Article Title: CB2R-mediated GPX4 stabilization: a new avenue for treating cognitive impairment in elderly migraine patients
    Article Snippet: .. Both samples were washed with phosphate-buffered saline (PBS), blocked with 5% bovine serum albumin (BSA) in PBS for 1 h, and incubated at 4 °C overnight with primary antibodies (ionized calcium-binding adapter molecule 1 (IBA-1), GPX4, interleukin-1β (IL-1β), CB2R, calcitonin gene-related peptide (CGRP), C-fos, synaptophysin (SYP), postsynaptic density protein 95 (PSD95), inducible nitric oxide synthase (iNOS), ubiquitin (UB), purchased from Cell Signaling Technology or Santa Cruz) in 3% BSA/PBS. .. After PBS washing, samples were incubated with secondary antibodies (rabbit 488, mouse 555: 1:500; Invitrogen) for 2 h at room temperature, followed by 4’,6-diamidino-2-phenylindole (DAPI) (1:2000; Thermo Fisher) staining for 10 min and PBS washes.

    Ubiquitin Proteomics:

    Article Title: CB2R-mediated GPX4 stabilization: a new avenue for treating cognitive impairment in elderly migraine patients.
    Article Snippet: .. Both samples were washed with phosphate-buffered saline (PBS), blocked with 5% bovine serum albumin (BSA) in PBS for 1 h, and incubated at 4 °C overnight with primary antibodies (ionized calcium-binding adapter molecule 1 (IBA-1), GPX4, interleukin-1β (IL-1β), CB2R, calcitonin gene-related peptide (CGRP), C-fos, synaptophysin (SYP), postsynaptic density protein 95 (PSD95), inducible nitric oxide synthase (iNOS), ubiquitin (UB), purchased from Cell Signaling Technology or Santa Cruz) in 3% BSA/PBS. .. After PBS washing, samples were incubated with secondary antibodies (rabbit 488, mouse 555: 1:500; Invitrogen) for 2 h at room temperature, followed by 4’,6-diamidino-2-phenylindole (DAPI) (1:2000; Thermo Fisher) staining for 10 min and PBS washes.

    Article Title: CB2R-mediated GPX4 stabilization: a new avenue for treating cognitive impairment in elderly migraine patients
    Article Snippet: .. Both samples were washed with phosphate-buffered saline (PBS), blocked with 5% bovine serum albumin (BSA) in PBS for 1 h, and incubated at 4 °C overnight with primary antibodies (ionized calcium-binding adapter molecule 1 (IBA-1), GPX4, interleukin-1β (IL-1β), CB2R, calcitonin gene-related peptide (CGRP), C-fos, synaptophysin (SYP), postsynaptic density protein 95 (PSD95), inducible nitric oxide synthase (iNOS), ubiquitin (UB), purchased from Cell Signaling Technology or Santa Cruz) in 3% BSA/PBS. .. After PBS washing, samples were incubated with secondary antibodies (rabbit 488, mouse 555: 1:500; Invitrogen) for 2 h at room temperature, followed by 4’,6-diamidino-2-phenylindole (DAPI) (1:2000; Thermo Fisher) staining for 10 min and PBS washes.



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    A Representative images of DIV14 primary neurons, fixed and stained with Histone H1, MAP2, <t>PSD95,</t> Synaptotagmin 1, METTL3, YTHDF1, and FTO in Wt and aSyn Tg neurons. Images are acquired using airy-scan at 63x magnification, scale bar = 10 µm. B Box and whisker plot representing the colocalisation of m6A regulators in Wt and aSyn Tg DIV14 primary neurons at the pre-and post-synapse. METTL3 colocalises with the post-synapse, (PCC, Wt = 0.810, aSyn Tg = 0.748). A significant difference (*, p-adj. value = 0.018) was observed between METTL3 at the post-synapse in Wt neurons when compared with aSyn Tg neurons. In the plots, the solid squares represent the pre-synaptic terminal and the solid circles represent post-synaptic terminal. C Box and whisker plot representing the colocalisation of m6A regulators at the pre-and post-synapse in Wt and aSyn Tg primary neurons. METTL3 colocalisation was significantly reduced in aSyn Tg neurons when compared with Wt at the post-synapse. Here, NC stands for negative control. In the plots, solid squares represent the Wt animals and solid circles represent the aSyn Tg animals. D Representative zoomed-in images of colocalization of METTL3 with post-synaptic maker, PSD95. The white arrows indicate the colocalisation of protein signal. Scale bar = 5 µm. Three replicate experiments were performed for Wt and aSyn Tg primary neuronal cultures and a minimum of 10–12 frames were acquired per experiment.
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    Image Search Results


    A Representative images of DIV14 primary neurons, fixed and stained with Histone H1, MAP2, PSD95, Synaptotagmin 1, METTL3, YTHDF1, and FTO in Wt and aSyn Tg neurons. Images are acquired using airy-scan at 63x magnification, scale bar = 10 µm. B Box and whisker plot representing the colocalisation of m6A regulators in Wt and aSyn Tg DIV14 primary neurons at the pre-and post-synapse. METTL3 colocalises with the post-synapse, (PCC, Wt = 0.810, aSyn Tg = 0.748). A significant difference (*, p-adj. value = 0.018) was observed between METTL3 at the post-synapse in Wt neurons when compared with aSyn Tg neurons. In the plots, the solid squares represent the pre-synaptic terminal and the solid circles represent post-synaptic terminal. C Box and whisker plot representing the colocalisation of m6A regulators at the pre-and post-synapse in Wt and aSyn Tg primary neurons. METTL3 colocalisation was significantly reduced in aSyn Tg neurons when compared with Wt at the post-synapse. Here, NC stands for negative control. In the plots, solid squares represent the Wt animals and solid circles represent the aSyn Tg animals. D Representative zoomed-in images of colocalization of METTL3 with post-synaptic maker, PSD95. The white arrows indicate the colocalisation of protein signal. Scale bar = 5 µm. Three replicate experiments were performed for Wt and aSyn Tg primary neuronal cultures and a minimum of 10–12 frames were acquired per experiment.

    Journal: NPJ Parkinson's Disease

    Article Title: The epitranscriptomic m6A RNA modification modulates the synapse in ageing and in a mouse model of synucleinopathy

    doi: 10.1038/s41531-026-01362-3

    Figure Lengend Snippet: A Representative images of DIV14 primary neurons, fixed and stained with Histone H1, MAP2, PSD95, Synaptotagmin 1, METTL3, YTHDF1, and FTO in Wt and aSyn Tg neurons. Images are acquired using airy-scan at 63x magnification, scale bar = 10 µm. B Box and whisker plot representing the colocalisation of m6A regulators in Wt and aSyn Tg DIV14 primary neurons at the pre-and post-synapse. METTL3 colocalises with the post-synapse, (PCC, Wt = 0.810, aSyn Tg = 0.748). A significant difference (*, p-adj. value = 0.018) was observed between METTL3 at the post-synapse in Wt neurons when compared with aSyn Tg neurons. In the plots, the solid squares represent the pre-synaptic terminal and the solid circles represent post-synaptic terminal. C Box and whisker plot representing the colocalisation of m6A regulators at the pre-and post-synapse in Wt and aSyn Tg primary neurons. METTL3 colocalisation was significantly reduced in aSyn Tg neurons when compared with Wt at the post-synapse. Here, NC stands for negative control. In the plots, solid squares represent the Wt animals and solid circles represent the aSyn Tg animals. D Representative zoomed-in images of colocalization of METTL3 with post-synaptic maker, PSD95. The white arrows indicate the colocalisation of protein signal. Scale bar = 5 µm. Three replicate experiments were performed for Wt and aSyn Tg primary neuronal cultures and a minimum of 10–12 frames were acquired per experiment.

    Article Snippet: The cells were then incubated with the primary antibodies diluted in blocking solution (FTO (mouse, Abcam – ab92821, 1:1000), METTL3 (rabbit, Cell Signalling - #96391S , 1:1000, RRID: AB_2800261), YTHDF1 (rabbit, Proteintech 17479-1-AP, 1:1000), MAP2 (rabbit, Proteintech – #17490-1-AP, 1:1000), postsynaptic density protein 95 (PSD95) (mouse, Synaptic systems 124011, 1:1000), PSD95(D74D3) (rabbit, Cell Signalling - #3409, 1:1000), synaptotagmin 1 (Syn1) (mouse, Synaptic systems - #105311, 1:1000) synaptotagmin1 (rabbit, Synaptic systems – 105102/3, 1:1000)) overnight at 4 °C, with constant shaking at a speed of 40–50 rpm.

    Techniques: Staining, Whisker Assay, Negative Control

    A Representative immunofluorescent images of DIV14 primary neurons stained with pre- (Syn1, magenta) and post-synaptic (PSD95, green) markers and MAP2 in red. Acquired at 63x magnification, airy scan mode. Scale bar = 10 µm. B Scatter plot for synapse counting for Wt and aSyn Tg mouse models with a significant reduction in the number of synapses (*, adj. p value = 0.044) in aSyn Tg primary neurons when compared with Wt. Three experiments were carried out for Wt and aSyn Tg neuronal cultures each and 10–12 frames per experiment were acquired for analysis.

    Journal: NPJ Parkinson's Disease

    Article Title: The epitranscriptomic m6A RNA modification modulates the synapse in ageing and in a mouse model of synucleinopathy

    doi: 10.1038/s41531-026-01362-3

    Figure Lengend Snippet: A Representative immunofluorescent images of DIV14 primary neurons stained with pre- (Syn1, magenta) and post-synaptic (PSD95, green) markers and MAP2 in red. Acquired at 63x magnification, airy scan mode. Scale bar = 10 µm. B Scatter plot for synapse counting for Wt and aSyn Tg mouse models with a significant reduction in the number of synapses (*, adj. p value = 0.044) in aSyn Tg primary neurons when compared with Wt. Three experiments were carried out for Wt and aSyn Tg neuronal cultures each and 10–12 frames per experiment were acquired for analysis.

    Article Snippet: The cells were then incubated with the primary antibodies diluted in blocking solution (FTO (mouse, Abcam – ab92821, 1:1000), METTL3 (rabbit, Cell Signalling - #96391S , 1:1000, RRID: AB_2800261), YTHDF1 (rabbit, Proteintech 17479-1-AP, 1:1000), MAP2 (rabbit, Proteintech – #17490-1-AP, 1:1000), postsynaptic density protein 95 (PSD95) (mouse, Synaptic systems 124011, 1:1000), PSD95(D74D3) (rabbit, Cell Signalling - #3409, 1:1000), synaptotagmin 1 (Syn1) (mouse, Synaptic systems - #105311, 1:1000) synaptotagmin1 (rabbit, Synaptic systems – 105102/3, 1:1000)) overnight at 4 °C, with constant shaking at a speed of 40–50 rpm.

    Techniques: Staining